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HA005 - Hatton
Área: 3 - Fisiologia / Bioquimica / Farmacologia
Apresentação: 10/09 (Sexta-feira) - Horário: 08h30 - 10h00 - Sala: 7
Metabolomic identification of novel salivary biomarkers for diagnostic and monitoring Diabetes
Moura DV, Caixeta DC, Santos P, Martins MM, Goulart LR, Sabino-Silva R
Fisiologia - UNIVERSIDADE FEDERAL DE UBERLÂNDIA
Conflito de interesse: Não há conflito de interesse
The diagnostic and monitoring of glycemia is an invasive and painful in diabetes mellitus (DM).Consequently, the search for non-invasive diagnostic biomarkers is of great interest in DM. The limitation to use saliva in clinical settings can be related to the reduced chemical composition knowledge. Here, a characterization of the salivary metabolome is presented in a diabetic animal model. Fluid chromatography coupled to mass spectrometry with time-of-flight system were employed to spot the metabolites in rats saliva. Fifteen Wistar rats were divided in non-diabetic (ND), diabetic (D) and diabetic 6U-treated of insulin (D6U). DM was induced by an intraperitoneal injection (60 mg/kg) of streptozotocin (STZ). The animals were submitted to 28 days of diabetes, and on the 21st day, the insulin- or placebo-treatment was started (#CEUA 13/16). The glycemia and urinary glucose confirmed the diabetic state. The metabolic salivary profile identified hundreds of novel compounds with potential to discriminate diabetes. Principal Component Analysis and clustering indicates 3 main candidates as novel salivary metabolites for salivary screening of diabetes: N-Arachidonoyl tyrosine, 1-11-Eicosenoyl-Triacylglycerol and Dimethylphosphatidylethanolamine (22:5(7Z/24:1(15Z) HMDB, (ANOVA).
Altogether, this salivary metabolomic analysis indicates novel salivary metabolites candidates to be applied in salivary diagnostic salivary platforms for non-invasive diabetic diagnostic platforms and for the salivary monitoring tool during insulin treatment.
(Apoio: CAPES N° (#23038.014934/2020-59). | FAPEMIG N° (#APQ-02872-16) | INCT-TeraNano N° (465669/2014-0))
HA006 - Hatton
Área: 4 - Odontopediatria
Apresentação: 10/09 (Sexta-feira) - Horário: 10h30 - 12h00 - Sala: 7
Selective Outcome Reporting (SOR) in Pediatric Dentistry restorative treatment Randomized Clinical Trials - A systematic Review
Elagami RA, Tedesco TK, Pannuti CM, Seabra G, Braga MM, Mendes FM, Raggio DP
Odontopediatria e Ortodontia - UNIVERSIDADE DE SÃO PAULO - SÃO PAULO
Conflito de interesse: Não há conflito de interesse
We aimed to evaluate the prevalence of SOR in the randomized clinical trials focusing on restorative treatment in primary molars. We searched for RCTs protocol registries in the ClinicalTrials.gov and WHO platform up to April/2021. We included any study classified as an RCT, with two or more experimental arms that evaluated any restorative treatment on primary teeth. The registries that did not result in any publications were excluded. Our search strategy identified 294 registries and 31 publications were included for data extraction. We have also calculated the median of journals' impact factors. Chi-square test was performed with 5% significance level. Our results found that the prevalence of SOR was identified in 19 papers (61.3%). The most common reason was a discrepancy in the primary outcome timeframe (48.4%). Twenty-six (83.9%) of the trials were registered retrospectively. Also, a discrepancy in sponsorship was identified in 22 (71%) of the trials. SOR was associated with the discrepancy in the follow-up period (p=0.003), with no association with registration timing (p=0.948). The impact factor of the journals ranged from 0.213 - 5.79. We found SOR in 9 (29%) of the six high impact factor journals and 10 (32.3%) of the eight with low impact.
Consequently, we emphasized the importance of transparency in RCT focusing on restorative care in children, including properly prospective registration and declaration of any discrepancy in the final manuscript, helping to avoid spin in science.
(Apoio: CAPES N° 001)
HA007 - Hatton
Área: 4 - Odontopediatria
Apresentação: 10/09 (Sexta-feira) - Horário: 10h30 - 12h00 - Sala: 7
Probiotic: in vivo evaluation of the effect on progression of periapical lesions, through microscopic analysis
Araujo LDC, Segato RAB, Messora MR, Furlaneto FAC, Salvador SLS, Nelson-Filho P, Pucinelli CM, Silva LAB
UNIVERSIDADE DE SÃO PAULO - RIBEIRÃO PRETO
Conflito de interesse: Não há conflito de interesse
The aim of the present study was to evaluate the effect of the probiotic Bifidobacterium animalis subsp. lactis (B. lacti) HN019 in vivo, as an irrigation solution for root canals in rats' teeth with induced periapical lesion. The animals were divided in four groups, as follows: group I: healthy teeth; group II: periapical lesion; group III: 2.5% sodium hypochlorite; group IV: probiotic, in 7, 21, 42 days. The probiotic irrigating solution was formulated by adding the probiotic at 2.7x 109 CFU in water medium and 2.0% of carboxymethylcellulose. The root canals were irrigated on day 0 and days 3, 7, 14, 21, 28 and 35 days, after periapical lesion induction. The mandible samples were stained in HE and analyzed under optical microscopy to describe the characteristics of the pulp tissue and the apical and periapical regions, in parallel with the morphometric analysis of the extension of the lesion area, osteoclast marking (TRAP), immunohistochemistry to identify markers of the osteoclastogenesis (RANK, RANK-L and OPG) and microbiological analysis using the Brown & Brenn technique. The data were analyzed using software R, version 4.0.0, applying SAS 9.4 and Graph Pad Prism 5, with a significance level of 5%. The histopathological, immunohistochemical and microbiological analysis parameters in probiotic groups were significantly better when compared to groups without periapical lesion and 2.5% sodium hypochlorite (p<0.05).
Therefore, probiotic demonstrated a therapeutic potential on the progression of periapical injuries.
(Apoio: FAPESP N° 2018/22038-3)
HA008 - Hatton
Área: 4 - Ortodontia
Apresentação: 10/09 (Sexta-feira) - Horário: 10h30 - 12h00 - Sala: 7
A microtomographic and histological analysis of the induced orthodontic movement in obese rats
Horta KC, Paula-Silva FWG, Stuani MBS, Elias LLK, Flores Mir C, Graf D, Consolaro A, Matsumoto MAN
UNIVERSIDADE DE SÃO PAULO - RIBEIRÃO PRETO
Conflito de interesse: Não há conflito de interesse
To evaluate the effect of obesity over induced tooth movement (ITM) through computerized micro-tomography and histological analyzes in obesity-induced Wistar rats. Forty rats of approximately 125 g were randomly assigned into four groups according to obesity status and ITM duration (mesial movement of the left maxillary first molar for seven and fourteen days). Eighty hemi-maxillas were processed for histological and micro-CT examination. ITM was measured in the micro-CTs. The number of osteoclasts was analyzed through staining of enzyme tartrate-resistant acid phosphatase. Also, the presence or absence of focal hyalinization (FH), frontal bone resorption (FBR), and active root resorption (ARRC) were evaluated in sections stained with Hematoxylin-Eosin. Data were submitted to appropriate statistical analysis using Graph Pad Prism 5.0 and Stata13 (α=0.05) Obese animals showed higher rates of ITM after seven (p=0.037) and fourteen days (p≤0.001) than non-obese animals. A higher quantity of osteoclast was observed after seven days of ITM in obese animals (p=0.043). Low frequencies of FH were observed after seven days of ITM. After seven days of ITM high frequency of FBR and low frequency of ARRC were found in obese animals, while, medium frequencies of FBR and ARRC were seen in non-obese animals. Low and medium frequencies of FBR were observed in non-obese and obese animals respectively after fourteen days of ITM.
Obese rats showed significantly higher rates of molar movement and different morphological responses in the surrounding bone and periodontium to ITM.
(Apoio: CAPES | FAPESP)
HA009 - Hatton
Área: 5 - Materiais Dentários
Apresentação: 10/09 (Sexta-feira) - Horário: 10h30 - 12h00 - Sala: 7
Self-assembling peptide-containing electrospun scaffolds for mineralized tissue regeneration
Araújo IJS, Ferreira JA, Daghrery A, Andrade JSR, Puppin-Rontani RM, Bottino MC
Odontologia Restauradora - FACULDADE DE ODONTOLOGIA DE PIRACICABA
Conflito de interesse: Não há conflito de interesse
Combining scaffolds and bioactive molecules to mimic the extracellular matrix (ECM) is vital for mineralized tissue regeneration strategies. Electrospinning allows the fabrication of ECM-like scaffolds. Meanwhile, self-assembling peptide (SAP) P11-4 is applied to repair early caries lesions. Thus, to explore SAP effectivity on mineralized tissue regeneration, distinct concentrations of P11-4 were adsorbed in electrospun scaffolds. After scaffolds immersion in simulated body fluid, mineral precipitation was verified by Scanning Electron Microscopy (SEM). FTIR and XRD detected functional groups and crystalline phases, respectively. Cytocompatibility, mineralization, and gene expression assays were conducted using stem cells from human exfoliated deciduous teeth. In vivo bone regeneration was assessed by defects in rat calvaria. ANOVA and Tukey posthoc tests verified differences for cytocompatibility, mineralization, gene expression, and in vivo regeneration (α=0.05). SEM images indicated mineral precipitation. FTIR and XRD confirmed apatite-like formation and crystallization induced by SAP-containing scaffolds. SAP-containing scaffolds were cytocompatible, stimulated cells to mineralization, and upregulated bone-related genes' expression. Also, substantial in vivo bone regeneration was induced after eight weeks. Histological analyses evidenced homogeneous bone formation.
Finally, SAP-containing scaffolds are promising bioinspired constructs for mineralized tissue regeneration in the oral and craniofacial complex.
(Apoio: CAPES N° 88887.368381/2019-00 | NIH N° R01DE026578)
HA010 - Hatton
Área: 5 - Dentística
Apresentação: 10/09 (Sexta-feira) - Horário: 10h30 - 12h00 - Sala: 7
Dental trauma splints for mixed dentition - A finite element analysis of splint material, extension, missing teeth, and PDL representation
Vilela ABF, Soares PBF, Versluis A, Soares CJ
Dentística - UNIVERSIDADE FEDERAL DE UBERLÂNDIA
Conflito de interesse: Não há conflito de interesse
This study evaluated the effect on the mobility of splint materials and extensions for avulsed central incisor, stabilized with and without the adjacent incisor under horizontal and vertical loading with different periodontal ligament (PDL) conditions. Seventeen 3D models were created from a CBCT of a 7-year-old patient without erupted permanent upper lateral incisors. A new method was created to simulate the avulsion injury, 1000N palatal load was applied on the central incisor creating increased alveolus with 0.45mm of PDL thickness. Wire-composite splints with 0.9mm (WCS) or 1.0mm nylon-composite splints (NCS) were created with and without the adjacent upper central incisor and four splint extensions were simulated. PDL conditions were investigated, simulating liquefied PDL or PDL with silicon. Mobility was calculated under horizontal, intrusive and extrusive loading. The NCS allowed higher mobility than the WCS, irrespective of extension, PDL or load conditions. During horizontal loading, silicon-like PDL allowed around 0.2mm mobility of the avulsed tooth with the WCS, similar to the intact tooth, whereas liquefied PDL allowed 25% higher mobility with a WCS than NCS.
A 1.0mm NCS seems to be a good option for splinting avulsion injuries during mixed dentition. The NCS provided flexibility for PDL healing while maintaining stability, even when missing adjacent teeth. Extensions beyond directly adjacent teeth did not alter mobility with the NCS, but should still be considered extra protection in case of bond failure or exfoliation.
(Apoio: CAPES N° 001 | FINEP N° FAPEMIG | CNPq)
HA011 - Hatton
Área: 5 - Dentística
Apresentação: 10/09 (Sexta-feira) - Horário: 16h00 - 17h30 - Sala: 7
A novel graphene-catechol dental sealant: properties and perspectives
Pereira R, Rischka K, Lins RBE, Stamboroski S, Mainardi MCAJ, Aguiar FHB
Odontologia Restauradora - FACULDADE DE ODONTOLOGIA DE PIRACICABA
Conflito de interesse: Não há conflito de interesse
This study aimed to assess the particles dispersion (D), antibacterial action (A), bond strength (BS), fracture pattern (FP), Vickers nanohardness (N), indentation modulus (IM), indentation creep (IC) and elastic part of indentation work (E) of dental sealants. An experimental sealant was incorporated by graphene (G) and graphene modified by L-dopa (Gd) in 0 and 0.25 concentrations (wt%). The D was assessed visually and microscopically. For A test, samples (n=10) were incubated with S.mutans and L.casei. Aliquots were spread on agar-plates. Colonies Forming Units (CFU) were quantified. For BS test, enamel surfaces of bovine teeth (n=8) were restored with sealant pillars, according to the groups. Pillars were submitted to microshear test. FP was quantified. For mechanical tests, samples (n=8) were assessed in a mechanical analyzer initially (T0) and after 10 days of water storage (T1). Data were statistically analyzed using One-way or Two-way ANOVA and Bonferroni tests. Gd was better dispersed than G. Control presented the highest number of S.mutans CFU. L.casei growth decreased as follows: Control > G > Gd. The BS was similar among groups. All groups showed mainly enamel-sealant adhesive FP. In general, at T0 and T1, Gd presented the highest N and IM, while Control obtained the highest IC, and both G and Gd had the highest E. From T0 to T1 only Control had its NH decreased. Other properties were not affected.
Gd sealant is a promising material, as it presents antibacterial activity, adequate bond strength, high mechanical strength, and still elasticity to absorb tensions.
(Apoio: CAPES N° 88887.368696/2019-00 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e. V.)
HA012 - Hatton
Área: 6 - Prótese
Apresentação: 10/09 (Sexta-feira) - Horário: 14h00 - 15h30 - Sala: 7
Mechanical behavior of different core and veneer designs for all-ceramic crowns: 3D Finite element analysis
Carvalho ABG, Andrade GS, Tribst JPM, Ramos NC, Luz JN, Borges ALS, Saavedra GSFA
Odontologia Restauradora - INSTITUTO DE CIÊNCIA E TECNOLOGIA / ICT-UNESP-SJC
Conflito de interesse: Não há conflito de interesse
To evaluate the mechanical behavior of different core and veneer designs for all-ceramic crowns. Using the finite element analysis (FEA) three designs were considered: CC - Conventional bilayer crown; MCV - Modified cutback core with ceramic veneer; MCCV - Modified cutback core with cemented machined ceramic veneer. Using a modeling software (Rhinoceros 6.0SR8), a superior central incisor model was created with a full-crown preparation and replicated to receive the three crown designs. Lithium disilicate (E=102.8 GPa) was the core material for all situations, fluorapatite (E=68 GPa) (CC/MCV) and leucite (E=65.8 GPa) (MCCV) ceramics were simulated as veneer materials. Models were exported to a FEA software to evaluate the stress distribution on crowns. All interfaces were considered bonded and materials considered isotropic, homogeneous, and linearly elastic. A 400 N load was applied at 45°, at 1 mm distant from the incisal edge and Maximum Principal Stress (MPa) criteria was used to evaluate the results. MCCV presented a more uniform stress distribution, while CC and MCV presented higher stress concentration in critical areas. The highest stress peak was on core of CC model (70.1 MPa). For veneer layer, the peaks were around 19 MPa. MCV core presented tensile stress peaks of 65.9, and veneer of 17.9 MPa. For MCCV, the peak in the core was 68.4 MPa with lower values in veneer layer (15.1 MPa).
The new all-ceramic crown design with a cemented veneer in modified core seems to be a promising alternative, improving the mechanical behavior and reducing stress on central incisors.
(Apoio: FAPESP N° 2020/07693-5)
HA013 - Hatton
Área: 7 - Estomatologia
Apresentação: 10/09 (Sexta-feira) - Horário: 14h00 - 15h30 - Sala: 7
Salivary proteomic predictors of radiotherapy-induced toxicities in oral cancer patients
Palmier NR, Ribeiro ACP, Brandão TB, Rossi T, Lopes MA, Migliorati CA, Paes Leme AF, Silva ARS
Diagnóstico Oral - FACULDADE DE ODONTOLOGIA DE PIRACICABA
Conflito de interesse: Não há conflito de interesse
Great medical advances have been achieved through personalized, molecular-based diagnosis and treatment. Therefore, this study tested the hypothesis that targeted proteins are associated with oral toxicities severity in oral and oropharyngeal squamous cell carcinoma (OSCC) patients undergoing radiotherapy (RT). Liquid biopsy was performed by subjecting whole saliva of 35 advanced OSCC patients to targeted proteomic analysis through selected reaction monitoring-mass spectrometry (SRM-MS) including 56 targeted proteins, and their abundance was correlated with clinical outcomes (Mann-Whitney and ANOVA with post-hoc Tukey HSD tests). Xerostomia, dysphagia, odynophagia, dysgeusia and oral candidiasis (OC) were daily evaluated using the National Cancer Institute Common Terminology Criteria for Adverse Events grading system (NCI CTCAE, Version 4.0, 2010) and the visual analogue scale. Overall, 90% of patients developed RT-induced oral toxicities and SRM-MS results indicated that 27 proteins, mainly related to biological processes such as innate immune responses, inflammatory response, cell migration, peptidase inhibitor activity, and iron coordination, were correlated with more severe grades (p<0.05) of dysgeusia, dysphagia, xerostomia, odynophagia, and OC in 68%, 60%, 43%, 34%, and 26% of patients, respectively.
This study originally demonstrates salivary proteomic predictors of debilitating RT-induced acute oral toxicities in late-stage OSCC patients, improving clinical protocols for personalized supportive care in Oncology.
(Apoio: FAPs - FAPESP N° 2018/04657-8)
HA014 - Hatton
Área: 7 - Estomatologia
Apresentação: 10/09 (Sexta-feira) - Horário: 14h00 - 15h30 - Sala: 7
Photobiomodulation therapy in Burning Mouth Syndrome: a randomized double-blind controlled trial
Marotta BM, Freitas GDH, Hanna R, Gallo CB
Diagnóstico Oral - UNIVERSIDADE DE SÃO PAULO - SÃO PAULO
Conflito de interesse: Não há conflito de interesse
Burning Mouth Syndrome (BMS) is characterized by a burning sensation despite the absence of clinical signs and topical clonazepam is considered the gold-standard therapy. This randomized double-blind controlled trial compared the effect of photobiomodulation therapy (PBM) and topical clonazepam for symptom relief in BMS patients. Fifteen patients were eligible and randomly allocated in the intervention or control group. The intervention group underwent 6 sessions of PBM with infrared low power laser (880nm, 90mW, 6J/cm2), which was simulated in the control group turning down the device and reproducing its signaling sounds. The control group rinsed 5 ml of 0.5mg/ml clonazepam solution 3 times a day for 21 days, simulated in the intervention group by rinsing a solution without clonazepam, to ensure the blindness of the received intervention. All patients were evaluated according the IMMPACT-II recommendations for outcome assessment in pain clinical trials, by a blinded research, at the baseline, end of treatment, 30 and 60 days of follow-up. Both treatments offered a significant symptom relief in BMS patients, according the numerical rating scale, without a significant difference between them. PBM provided an earlier effect, maintained for at least 30 days. And topical clonazepam had a slightly higher long-term effect at the 60 day follow-up at other outcomes measures, such as brief pain inventory and oral related quality of life.
PBM and topical clonazepam are effective in controlling pain symptom in BMS patients.
(Apoio: CAPES N° 001)