Using point coordinates to measure root canal curvature in periapical radiograph and CBCT images using free software
Gialain IO, Yanaguizawa WH, Fontana CE, Caldeira CL, Cavalcanti MGP
Ciências Odontológicas Integradas - UNIVERSIDADE DE CUIABÁ
Conflito de interesse: Não há conflito de interesse
This study proposed to demonstrate a technique to calculate root canal curvature on 2D and 3D images using point coordinate, named Point Method (PM), and further compare these methods to the most used method (Schneider). The total sample comprised 120 teeth (30 upper lateral incisors, 30 lower premolars, 30 upper molars, and 30 lower molars), with no radiopaque material on the root canal or pulp chamber. Each tooth underwent periapical radiograph and CBCT exams, and the curvature was analyzed by three different methods - Schneider, 2D PM, and 3D PM. Schneider and 2D PM were performed on periapical radiograph and 3D PM on CBCT. Point Method analysis was made placing points on the canal lumen with a vertical distance of 1mm among points. Using open-source software, the coordinates for each point were transferred into a spreadsheet with analytical geometry formulas. A repeated measures test was made to compare angular values among the methods and an independent measures test was performed to compare the dental groups and the root thirds. The mean curvature angle values found using 3D PM (89.01°) were significantly higher when compared to 2D PM (56.72°) and Schneider (34.62°) for all dental groups. Multirooted teeth had a greater mean curvature angle than single-rooted and the apical third also presented the most curved root canals amongst the root thirds. The results presented herein show that 3D curvature was higher when compared with 2D analysis. Higher curvature angle values were found at the apical third and on multirooted teeth. (Apoio: CAPES N° 1570958)HA016 - Hatton
Área:
8 - Periodontia
Periodontal Collapse and Incipient Vascular Abnormalities forming a cluster at the end of 2nd decade of life: a population-based study
Costa CM, Costa SA, Souza SFC, Alves CMC, Thomaz EBAF, Silva AAM, Ribeiro CCC
Programa de Pós-graduação Em Odontologia - UNIVERSIDADE FEDERAL DO MARANHÃO
Conflito de interesse: Não há conflito de interesse
Periodontitis and cardiovascular disease continuums are associated with each other in late adulthood; we hypothesize this cluster may start as early as adolescence. Thus, we explored the association between initial periodontal collapse and incipient vascular abnormalities at the end of 2nd decade of life. Population-based sample from RPS Birth Cohort Consortium, follow-up at 18-19 years, São Luís (n=2515). The theoretical model explored the complex structure between Initial Periodontitis and the Cardiovascular Risk, adjusted to socioeconomic status, smoking, alcohol, and obesity, through Structural Equation Modelling. The Cardiovascular Risk was a latent variable representing the shared variance of carotid-femoral pulse wave velocity, systolic and diastolic blood pressures. Initial Periodontitis representing the shared variance of visible plaque index, bleeding on probing, probing depth ≥4mm, and clinical attachment loss ≥3mm. Socioeconomic Status reduced the Initial Periodontitis (Standardized Coefficient -SC: -0.132; p<0.001) and the Cardiovascular Risk values (SC: 0.069; p= 0.032); Obesity increased Initial Periodontitis (SC: 0.111; p=0.001) and Cardiovascular Risk values (SC: 0.390; p=<0.001). The Cardiovascular Risk and Initial Periodontitis associations were bidirectional ones (SC= 0.079; p=0.032). The initial periodontal collapse and incipient vascular abnormalities in young formed a cluster. The attempt to reduce the diseases continuums should begin much early and direct to their common risk factors. (Apoio: CNPq)HA017 - Hatton
Área:
8 - Periodontia
Protease-activated receptor 1 (PAR1) enhances ectopic bone formation in human periodontal ligament stem cell (hPDLSC) sheets in vivo
Alves T, Gasparoni LM, Balzarini D, Albuquerque-Souza E, Rovai ES, Mendoza AAH, Sipert CR, Holzhausen M
UNIVERSIDADE DE SÃO PAULO - SÃO PAULO
Conflito de interesse: Não há conflito de interesse
We evaluated the osteogenic outcomes underlying PAR1 activation and how it affects bone formation in hPDLSC sheets in vivo. Briefly, hPDLSCs were obtained from 3 patients. Groups were: control, osteogenic and osteogenic + PAR1 activation by TFLLR-NH2 for 2, 7 and 14 days. PAR1 osteogenic potential pathways Wnt/β-catenin, TGF-βRI, MEK, p38 MAPK, and FGFR/VEGFR were assessed after blockage. Cell phenotype was determined by flow cytometry and immunofluorescence. Colony-forming-unit and cell proliferation assays were performed. qRT-PCR for Alkaline Phosphatase (ALP), β-galactosidase, Collagen I (COL1), Osteocalcin (OC), Osteoprotegerin (OPG), Osterix, PAR1, Periostin (POSTN), RANKL and RUNX2 was conducted. ELISA was performed to COL1, OC, OPG, PAR1, POSTN and RANKL. Calcium deposition was quantified through ALP and Alizarin Red Staining. Cell sheet microstructure was analyzed through H&E, light and scanning electron microscopy. In vivo, 14-day pre-induced hPDLSC sheets from the 3 groups were transplanted to Balb/c nude mice and evaluated after 60 days. Immunohistochemistry for bone sialoprotein (BSP), integrin β1, COL1 and histological stains (H&E, Van Giesson, Masson's Trichrome and Von Kossa) was carried out. Ectopic bone formation was evaluated through micro-CT. One-way ANOVA followed by Tukey's test (P value of 0.05) was used. PAR1 activation significantly increased pro-osteogenic gene and protein expression, calcium deposition and enhanced ectopic bone formation in vivo. PAR1 plays a major role in enhancing bone formation in vitro and in vivo. (Apoio: FAPESP N° 17/23158-0 | FAPESP N° 18/13818-5)HA018 - Hatton
Área:
8 - Periodontia
Association between poor oral health and adverse COVID-19 outcomes in hospitalized patients
Costa CA, Vilela ACS, Oliveira SA, Leles CR, Costa NL
Periodontia - UNIVERSIDADE FEDERAL DE GOIÁS
Conflito de interesse: Não há conflito de interesse
This study aimed to assess the dental and periodontal status of hospitalized COVID-19 patients and their association with the incidence of adverse COVID-19 outcomes. 128 hospitalized patients with COVID-19 diagnosis were included. DMFT index, periodontal status, and tooth loss patterns (Eichner index) were assessed by means of in-hospital clinical examination. Associations between oral health measures, the severity of COVID-19 symptoms, and hospitalization endpoints were tested using chi-square test and prevalence ratio (PR) estimation using Generalized Linear Model (GLM) with log-Poisson regression with robust error variances. Oral health-related variables, comorbidities, and patient's age were included in the regression models using a block-wise selection of predictors. Poor oral health conditions were highly prevalent and associated with critical COVID-19 symptoms, higher risk for admission in the intensive care unit, and mortality. Periodontitis was significantly associated with ICU admission (PR=1.44; p=0.017), critical symptoms (PR=2.56; p=0.002), and risk of death (PR=2.02; p=0.028) when adjusted for age and comorbidities. Eichner index (B and C) was associated with ICU admission, probably affected by the relationship between advanced age and tooth loss. The positive association between deleterious oral health-related conditions, especially periodontitis, and serious COVID-19 outcomes suggest that dental and periodontal status may play a role in the prognosis of hospitalized COVID-19 patients. (Apoio: FAPEG N° #CVD2020051000009)HA019 - Hatton
Área:
10 - Implantodontia básica e biomateriais
Tailoring Cu-loaded electrospun membrane with antibacterial ability for guided bone regeneration
Cordeiro JM, Avila ED, Yang F, Beucken JD, Barão VAR
Odontologia - CENTRO UNIVERSITÁRIO DAS FACULDADES ASSOCIADAS DE ENSINO
Conflito de interesse: Não há conflito de interesse
We tailored copper (Cu)-loaded electrospun membranes for guided bone regeneration (GBR) targeting the stimulation of innate cells active in bone growth and the prevention of treatment-related infections. Functional GBR membranes were produced via an electrospinning set-up using a silk-based solution in association with polyethylene oxide (Silk:PEO - control). Experimental groups were loaded with Cu oxide (CuO 1%, CuO 0.5%, CuO 0.1%, and CuO 0.05%). The morphological, structural, chemical and mechanical properties of membranes were evaluated. Direct and indirect in vitro cytocompatibility experiments were performed with human bone mesenchymal stem cells and human umbilical vein endothelial cells. The antibacterial potential of membranes was tested with Staphylococcus aureus biofilm (24 h). One-way ANOVA and Tukey's HSD test were used for multiple comparisons (p<0.05). CuO was successfully incorporated into membranes as clusters without affecting its mechanical resistance. Cu ions were released from the nanofibers over 1 week, being expressively greater in an acidic condition. Increased Cu concentration produced membranes with thinner nanofiber, greater porosity, and stronger antimicrobial effect (p<0.01). CuO 0.1% and CuO 0.05% were able to support and stimulate cells adhesion and proliferation (p<0.05), as well as angiogenesis. The tailored functional Cu-loaded GBR membrane with combined cell stimulatory behavior and antibacterial capacity may be a valuable strategy to diminish the risk of infections and improve the regeneration process. (Apoio: FAPs - Fapesp N° 2018/14117-0 | CNPq N° 304853/2018-6 | CAPES N° Finance Code 001)HA020 - Hatton
Área:
10 - Implantodontia básica e biomateriais
Titanium with nanotopography inhibits osteoclast-induced disruption of osteoblast differentiation by regulating histone methylation
Bighetti-Trevisan RL, Almeida LO, Gordon J, Tye C, Lian JB, Stein GS, Rosa AL, Beloti MM
Biologia Básica e Oral - UNIVERSIDADE DE SÃO PAULO - RIBEIRÃO PRETO
Conflito de interesse: Não há conflito de interesse
The aim of this study was to evaluate the regulation of the osteoclast/osteoblast crosstalk by titanium with nanotopography (Ti Nano) and the mechanism involved in this process. Osteoblasts were cultured on Ti Nano and Ti Machined and osteoclasts into inserts in a co-culture model for 48 h, and non-cocultured osteoblasts were used as control. The RNAseq (DESeq2: FC>1.7; p≤0.05) detected that osteoclasts downregulated the expression of genes related to osteogenesis and upregulated genes related to histone modification and chromatin organization in osteoblasts grown on both surfaces. Osteoclasts also inhibited (p≤0.05) the gene expression of Runx2, Dlx5, Alpl, Ibsp, Bglap and Opg and protein expression of RUNX2 and ALPL, and such effect was reduced by Ti Nano. Regarding the mechanism, an increase in H3K9me2, H3K27me3 and EZH2 protein expression was observed on both Ti surfaces under coculture conditions (p≤0.05). ChIP assay revealed accumulation of H3K9me2 repressing the promoter region of Alpl and H3K27me3 repressing Runx2, Alpl, Ibsp and Opg in osteoblasts in the presence of osteoclasts, which were attenuated by Ti Nano. Immunofluorescence corroborated the ChIP assay, exhibiting less RUNX2 and ALPL and more H3K27me3 staining in osteoblasts under coculture conditions in a less pronounced way on Ti Nano. In conclusion, Ti Nano may favor the clinical outcomes of implant osseointegration, since this nanotopography inhibited the osteoclast-induced disruption of osteoblast differentiation by reducing the accumulation of H3K9 and H3K27 methylated histones. (Apoio: FAPESP N° 2017/23888-8 | FAPESP N° 2018/17356-6 | CNPq N° 303464/2016-0)PN0002 - Painel Aspirante
Área:
1 - Cirurgia bucomaxilofacial
Avaliação tridimensional da via aérea superior após cirurgia ortognática em pacientes classe II e classe III de Angle
Domingues NRAP, Salmen MD, Salmen FS, Sampaio RMF, Penna LAP, Brandt WC, Sendyk WR, Roman-Torres CVG
Odontologia - UNIVERSIDADE SANTO AMARO
Conflito de interesse: Não há conflito de interesse
O tratamento de mal oclusão por vezes exige a realização de cirurgia ortognática com alvo em corrigir deficiências dento-esqueléticas, restabelecer a harmonia facial e a oclusão. O objetivo deste estudo observacional tipo coorte foi avaliar tridimensionalmente (3D) o volume e área mínima axial das vias aéreas superiores (VAS) após 30 dias dos procedimentos cirúrgicos ortognáticos realizados em pacientes com mal oclusão tipo II e III. A amostra foi proveniente da análise dos prontuários clínicos de pacientes submetidos à cirurgia entre os anos de 2017 e 2019. Entre os 50 prontuários analisados, 16 foram excluídos da pesquisa por não preencherem os critérios de elegibilidade. Foram realizadas as mensurações de: volume total e área axial mínima da via aérea superior. Análise das tomografias computadorizadas (TC) de pacientes submetidos à cirurgia avaliados nos momentos pré-operatório (T1) e pós-operatório de até 30 dias (T2), por meio do software Dolphin Imaging®. Os pacientes submetidos à cirurgia ortognática para classe II apresentaram diferença significativa após a execução da terapêutica cirúrgica com aumento dos valores em área total e área mínima axial, diferente dos pacientes classe III, os quais não apresentaram diferença significativa. A correlação entre assimetria mandibular e morfologia das VAS raramente é estudada, neste estudo os indivíduos classe II apresentaram um aumento significativo no volume da VAS, é compulsório saber as reações favoráveis e desfavoráveis destes pacientes a longo prazo e planejar as abordagens de forma multidisciplinar.PN0003 - Painel Aspirante
Área:
1 - Cirurgia bucomaxilofacial
Concordância entre diagnóstico clínico-radiográfico e histopatológico em lesões fibro-ósseas maxilo-faciais
Melo KDCM, Porto DE, Diniz JA, Barbirato DS, Andrade RRA, Andrade ESS
Programa de Pós-graduação Em Odontologia - UNIVERSIDADE DE PERNAMBUCO
Conflito de interesse: Não há conflito de interesse
Esta pesquisa teve por objetivo investigar as características clínicas, radiográficas e histopatológicas relacionadas ao diagnóstico de lesões fibro-ósseas (LFOs) dos maxilares. Estudo baseado em coleta sistemática de dados, realizado no Laboratório de Patologia Cirúrgica da Faculdade de Odontologia de Pernambuco, Brasil. Noventa e seis casos de LFOs foram documentados, entre os quais, casos de Displasia Fibrosa (DF), Displasia Cimento-óssea (DCO) e Fibroma Ossificante (FO) em um período de 12 anos. Os dados foram obtidos para análise pelo teste exato de Fisher, considerando uma significância de 5% (p ≤ 0,05). A concordância entre os diagnósticos clínico-radiográfico e histopatológico foi avaliada pelo teste Kappa. Lesões mistas radiolúcidas e radiopacas foram as mais prevalentes, sendo DCO e DF, respectivamente. Os aspectos radiográficos dos LFOs estudados não diferiram significativamente (p= 0,09). A ocorrência de diferentes LFOs diagnosticados por exame histopatológico diferiu significativamente entre a mandíbula e a maxila (p <0,05). A concordância observada entre o diagnóstico clínico-radiográfico e o histopatológico foi classificada como moderada (Kappa = 0,5). LFOs dos maxilares representam um grupo de lesões em que o estabelecimento do diagnóstico clínico e radiográfico apoiado pela confirmação histopatológica é crítico e desafiador. FO foi o diagnóstico com maior percentual de concordância. Ressalta-se a necessidade de mais estudos que abordem o assunto, a fim de otimizar o diagnóstico e tratamento dos LFOs. (Apoio: CAPES)PN0004 - Painel Aspirante
Área:
1 - Cirurgia bucomaxilofacial
Avaliação microtomográfica de métodos para prevenção da osteonecrose dos maxilares induzida por medicamentos
Hadad H, de Jesus LK, Rodrigues LGS, Santos AFP, Matheus HR, Almeida JM, Okamoto R, Souza FA
Clínica e Cirurgia - UNIVERSIDADE ESTADUAL PAULISTA - ARAÇATUBA
Conflito de interesse: Não há conflito de interesse
Avaliar o efeito de terapias preventivas na osteonecrose dos maxilares induzida por medicamentos, no processo de reparo alveolar. Foram utilizados 72 ratos wistar, tratados com 0,035 mg/kg de ácido zoledrônico e posteriormente submetidos a exodontia dos molares inferiores direitos. Após a exodontia, os animais foram divididos em 9 grupos (n=8), GS (Sham - não recebeu zolendronato), GC (Coágulo), Gβ (β-tricálcio-fosfato), GD (gel de doxiciclina a 10%), GP (terapia foto-dinâmica), GDβ, GPβ, GPD, e GPDβ. Após 28 dias da exodontia, realizou-se escaneamento das peças em microtomógrafo Skyscan para obtenção do volume ósseo (BV/TV), espessura do trabeculado (Tb.Th), separação (TB.Sp) e número (Tb.N) de trabéculas. Os dados foram submetidos a análise estatística (one-way ANOVA, seguido tukey's post hoc, p <0.05). Todos os grupos apresentaram maiores médias de BV/TV quando comparados a GS (42,17% ± 2,65) e o melhor resultado foi de GPDB, 69,85% ± 6,25, seguido por GPD e GDB (64,62 ±0,13 e 64,69 ± 4,40), quando comparado ao GC, GS, GP e GPB (P <0,05). Em Tb.Th, GD apresentou 0,163 mm ± 0,015 (P =0,043) quando comparado GP, 0,111mm ± 0,0070. Quanto ao Tb.N, GPD 5,53 mm ± 5,53 apresentou maior numero de trabéculas, comparado a GS, 3,68mm ± 3,48 (P <0,05). Não houve diferença para Tb.Sp (P =0,199). Qualitativamente, GS demostrou sequestros ósseos, enquanto as terapias permitiram o processo de reparo. As terapias associadas ou não demonstram ser efetivas na prevenção da osteonecrose e o uso de biomaterial e doxiciclina, demostrou manutenção do volume alveolar. (Apoio: FAPs - FAPESP N° 2019/22657-8)PN0005 - Painel Aspirante
Área:
1 - Biologia craniofacial
Avaliação da reparação óssea em calvária de coelhos após cirurgia com uso de elastina recombinante (ELRs)
Paulini MR, Ruggieri INC, Fonseca AI, Macedo AP, Alonso M, Rodríguez C, Issa JPM, Feldman S
Reabilitação Oral - UNIVERSIDADE DE SÃO PAULO - RIBEIRÃO PRETO
Conflito de interesse: Não há conflito de interesse
ELRs (elastin-like recombinamers) estão sendo estudadas para regeneração tecidual. O objetivo do presente estudo foi a avaliação de fatores osteogênicos sobre os efeitos da ELRs no reparo ósseo em defeitos cirúrgicos através da técnica de cirurgia em cálvarias de coelhos. Foram usados 5 coelhos, sendo que na mesma calvária de cada coelho foram realizados dois defeitos cirúrgicos (lado Direito da calvária-Grupo Controle e o lado Esquerdo da calvária-Grupo tratado com ELRs). As amostras das calvárias foram destinadas a análise de tomografia computadorizada e análises histológicas para quantificação de tecido ósseo, fibras colágenas, vasos sanguíneos e tecido adiposo. Foi realizada análise estatística dos dados histológicos através dos testes de Kolmogorov-Smirnov e Shapiro-Wilk. Para a análise tomográfica foi realizado o teste de Wilcoxon. Não foi encontrada diferença significante para o tecido adiposo entre os grupos (p=0,836). Para as demais varáveis foi encontrada diferença significante entre os grupos (Fibras p=0,001; Vasos p<0,001; Tecido neoformado p=0,001).Na análise tomográfica foi encontrada diferença significante entre os grupos (p<0,01). A ERLs contribuiu positivamente na regeneração tecidual, dados comprovados através histologia e também pelos dados da tomografia. A análise histológica quantitativa de tecido adiposo demonstrou a biocompatibilidade da ERLs devido a ausência de diferença encontrada nos resultados. Portanto, o biomaterial estudado apresenta capacidade osteogênica e biocompatibilidade. (Apoio: CNPq N° 149981/2019-8)