PN0684 - Painel Efetivo
Área: 3 - Fisiologia / Bioquimica / Farmacologia

Salivary IgA concentration in Prader Willi syndrome. Preliminary study
Ferrary T, Bianchi L, Armada M, Echaide M, Orman B
Medicina Interna - UNIVERSIDAD DE BUENOS AIRES
Conflito de interesse: Não há conflito de interesse

Prader Willi Syndrome (PW) is a rare genetic disorder. The oral characteristics are: xerostomia, thick and viscous saliva and dental amelogenesis. The objective of the present study was to investigate the presence of immunoglobulins in the total saliva of patients diagnosed with Prader Willi (PW). Eight individuals with PW and 8 healthy individuals (CON) aged 6 to 18 years participated in this study. Total unstimulated saliva was collected and centrifuged. The supernatants were separated and lyophilized. Salivary pH was measured and immunoglobulins were analyzed using the ELISA technique. Results: Salivary pH (CON, 7.19 ± 0.45; PW, 6.95 ± 0.43) did not differ between groups (p = 0.182). Measurable levels of IgG, IgA, IgM and IgE were detected in all patients. IgG levels (CON, 1,058 ± 0.386; PW, 1,067 ± 0.373; p = 0.001), IgM (CON, 0.683 ± 0.147, PW, 0.646 ± 0.161; p = 0.561) and IgE levels (CON, 1,241 ± 0.378; PW, 1,312 ± 0.412; p = 0.664) did not present differences between the groups, while the levels of IgA (CON, 0.915 ± 0.187; PW 0.805 ± 0.235; p = 0.362) presented a decrease with respect to the controls.
The results of the present study suggest differences in Salivary IgA levels between individuals with and without PW could be related to the cariogenic level found in these patients.

---

PN0686 - Painel Efetivo
Área: 3 - Fisiologia / Bioquimica / Farmacologia

Lidocaine and IgGp induce apoptosis by activation of caspase 3 and 9 in rat atria
Orman B, Villarruel EQ, Pimentel AC, Taure MNS, Ferrary T
Farmacología - UNIVERSIDAD DE BUENOS AIRES
Conflito de interesse: Não há conflito de interesse

Lidocaine is used as local anesthetics in dentistry and is used for the treatment of ventricular arrhythmias by the decrease of the frequency and tension of contractions. Periodontal patients, autoantibodies (IgGp), that recognize the β1 cardiac receptor, were detected. The objective of this work was to study the influence of IgGp on the apoptotic effect promoted by L by the caspase-3(C3) and caspase-9 (C9) enzyme activity on the rat atrium. Three Wistar rat atria were used. Rat atrium incubations were performed in the presence of L, IgG from healthy patients (IgGn) and IgGp, alone and/or in the presence of ouabain (O), inhibitor of Na+-K+-ATPase; xamoterol (XAM), β agonist; and atenolol (A), β antagonist. C3 and C9 enzyme activity were measured by CaspASE Assay System Kit. Our results showed that lidocaine caused an increase in C3 and C9 enzyme activity in a concentration-dependent manner in the rat atrium (p <0.001). This effect was decreased in the presence of O 1x10-4M (p <0.001). Incubations with L+IgGp or L+XAM increased the effect on C3 and C9 enzyme activity and the addition of A 1x10-5M to L+IgGp incubations reversed the effect of IgGp. IgGn did not modify the activity of C3 and C9 induce L
L and IgGp promote an apoptotic effect in the rat atrium with an increased C3 and C9 enzyme activity. L and IgGp would activate different signaling pathways that involve the Na⁺/K⁺ pump (effect blocked by O) and the β1 receptors (effect blocked by A) on the isolated atrial myocardium, respectively; and that both would converge in the activation of the intrinsic pathway through caspases 3 and 9.

---