HA016 - Hatton
Área: 8 - Periodontia

Apresentação: 10/09 (Sexta-feira) - Horário: 16h00 - 17h30 - Sala: 7

Periodontal Collapse and Incipient Vascular Abnormalities forming a cluster at the end of 2nd decade of life: a population-based study
Costa CM, Costa SA, Souza SFC, Alves CMC, Thomaz EBAF, Silva AAM, Ribeiro CCC
Programa de Pós-graduação Em Odontologia - UNIVERSIDADE FEDERAL DO MARANHÃO
Conflito de interesse: Não há conflito de interesse

Periodontitis and cardiovascular disease continuums are associated with each other in late adulthood; we hypothesize this cluster may start as early as adolescence. Thus, we explored the association between initial periodontal collapse and incipient vascular abnormalities at the end of 2nd decade of life. Population-based sample from RPS Birth Cohort Consortium, follow-up at 18-19 years, São Luís (n=2515). The theoretical model explored the complex structure between Initial Periodontitis and the Cardiovascular Risk, adjusted to socioeconomic status, smoking, alcohol, and obesity, through Structural Equation Modelling. The Cardiovascular Risk was a latent variable representing the shared variance of carotid-femoral pulse wave velocity, systolic and diastolic blood pressures. Initial Periodontitis representing the shared variance of visible plaque index, bleeding on probing, probing depth ≥4mm, and clinical attachment loss ≥3mm. Socioeconomic Status reduced the Initial Periodontitis (Standardized Coefficient -SC: -0.132; p<0.001) and the Cardiovascular Risk values (SC: 0.069; p= 0.032); Obesity increased Initial Periodontitis (SC: 0.111; p=0.001) and Cardiovascular Risk values (SC: 0.390; p=<0.001). The Cardiovascular Risk and Initial Periodontitis associations were bidirectional ones (SC= 0.079; p=0.032).
The initial periodontal collapse and incipient vascular abnormalities in young formed a cluster. The attempt to reduce the diseases continuums should begin much early and direct to their common risk factors.
(Apoio: CNPq)

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HA017 - Hatton
Área: 8 - Periodontia

Apresentação: 10/09 (Sexta-feira) - Horário: 16h00 - 17h30 - Sala: 7

Protease-activated receptor 1 (PAR₁) enhances ectopic bone formation in human periodontal ligament stem cell (hPDLSC) sheets in vivo
Alves T, Gasparoni LM, Balzarini D, Albuquerque-Souza E, Rovai ES, Mendoza AAH, Sipert CR, Holzhausen M
UNIVERSIDADE DE SÃO PAULO - SÃO PAULO
Conflito de interesse: Não há conflito de interesse

We evaluated the osteogenic outcomes underlying PAR₁ activation and how it affects bone formation in hPDLSC sheets in vivo. Briefly, hPDLSCs were obtained from 3 patients. Groups were: control, osteogenic and osteogenic + PAR₁ activation by TFLLR-NH2 for 2, 7 and 14 days. PAR₁ osteogenic potential pathways Wnt/β-catenin, TGF-βRI, MEK, p38 MAPK, and FGFR/VEGFR were assessed after blockage. Cell phenotype was determined by flow cytometry and immunofluorescence. Colony-forming-unit and cell proliferation assays were performed. qRT-PCR for Alkaline Phosphatase (ALP), β-galactosidase, Collagen I (COL1), Osteocalcin (OC), Osteoprotegerin (OPG), Osterix, PAR1, Periostin (POSTN), RANKL and RUNX2 was conducted. ELISA was performed to COL1, OC, OPG, PAR1, POSTN and RANKL. Calcium deposition was quantified through ALP and Alizarin Red Staining. Cell sheet microstructure was analyzed through H&E, light and scanning electron microscopy. In vivo, 14-day pre-induced hPDLSC sheets from the 3 groups were transplanted to Balb/c nude mice and evaluated after 60 days. Immunohistochemistry for bone sialoprotein (BSP), integrin β1, COL1 and histological stains (H&E, Van Giesson, Masson's Trichrome and Von Kossa) was carried out. Ectopic bone formation was evaluated through micro-CT. One-way ANOVA followed by Tukey's test (P value of 0.05) was used. PAR₁ activation significantly increased pro-osteogenic gene and protein expression, calcium deposition and enhanced ectopic bone formation in vivo.
PAR₁ plays a major role in enhancing bone formation in vitro and in vivo.
(Apoio: FAPESP  N° 17/23158-0  |  FAPESP  N° 18/13818-5)

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HA018 - Hatton
Área: 8 - Periodontia

Apresentação: 10/09 (Sexta-feira) - Horário: 16h00 - 17h30 - Sala: 7

Association between poor oral health and adverse COVID-19 outcomes in hospitalized patients
Costa CA, Vilela ACS, Oliveira SA, Leles CR, Costa NL
Periodontia - UNIVERSIDADE FEDERAL DE GOIÁS
Conflito de interesse: Não há conflito de interesse

This study aimed to assess the dental and periodontal status of hospitalized COVID-19 patients and their association with the incidence of adverse COVID-19 outcomes. 128 hospitalized patients with COVID-19 diagnosis were included. DMFT index, periodontal status, and tooth loss patterns (Eichner index) were assessed by means of in-hospital clinical examination. Associations between oral health measures, the severity of COVID-19 symptoms, and hospitalization endpoints were tested using chi-square test and prevalence ratio (PR) estimation using Generalized Linear Model (GLM) with log-Poisson regression with robust error variances. Oral health-related variables, comorbidities, and patient's age were included in the regression models using a block-wise selection of predictors. Poor oral health conditions were highly prevalent and associated with critical COVID-19 symptoms, higher risk for admission in the intensive care unit, and mortality. Periodontitis was significantly associated with ICU admission (PR=1.44; p=0.017), critical symptoms (PR=2.56; p=0.002), and risk of death (PR=2.02; p=0.028) when adjusted for age and comorbidities. Eichner index (B and C) was associated with ICU admission, probably affected by the relationship between advanced age and tooth loss.
The positive association between deleterious oral health-related conditions, especially periodontitis, and serious COVID-19 outcomes suggest that dental and periodontal status may play a role in the prognosis of hospitalized COVID-19 patients.
(Apoio: FAPEG  N° #CVD2020051000009)

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HA019 - Hatton
Área: 10 - Implantodontia básica e biomateriais

Apresentação: 10/09 (Sexta-feira) - Horário: 16h00 - 17h30 - Sala: 7

Tailoring Cu-loaded electrospun membrane with antibacterial ability for guided bone regeneration
Cordeiro JM, Avila ED, Yang F, Beucken JD, Barão VAR
Odontologia - CENTRO UNIVERSITÁRIO DAS FACULDADES ASSOCIADAS DE ENSINO
Conflito de interesse: Não há conflito de interesse

We tailored copper (Cu)-loaded electrospun membranes for guided bone regeneration (GBR) targeting the stimulation of innate cells active in bone growth and the prevention of treatment-related infections. Functional GBR membranes were produced via an electrospinning set-up using a silk-based solution in association with polyethylene oxide (Silk:PEO - control). Experimental groups were loaded with Cu oxide (CuO 1%, CuO 0.5%, CuO 0.1%, and CuO 0.05%). The morphological, structural, chemical and mechanical properties of membranes were evaluated. Direct and indirect in vitro cytocompatibility experiments were performed with human bone mesenchymal stem cells and human umbilical vein endothelial cells. The antibacterial potential of membranes was tested with Staphylococcus aureus biofilm (24 h). One-way ANOVA and Tukey's HSD test were used for multiple comparisons (p<0.05). CuO was successfully incorporated into membranes as clusters without affecting its mechanical resistance. Cu ions were released from the nanofibers over 1 week, being expressively greater in an acidic condition. Increased Cu concentration produced membranes with thinner nanofiber, greater porosity, and stronger antimicrobial effect (p<0.01). CuO 0.1% and CuO 0.05% were able to support and stimulate cells adhesion and proliferation (p<0.05), as well as angiogenesis.
The tailored functional Cu-loaded GBR membrane with combined cell stimulatory behavior and antibacterial capacity may be a valuable strategy to diminish the risk of infections and improve the regeneration process.
(Apoio: FAPs - Fapesp  N° 2018/14117-0  |  CNPq  N° 304853/2018-6  |  CAPES  N° Finance Code 001)

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HA020 - Hatton
Área: 10 - Implantodontia básica e biomateriais

Apresentação: 10/09 (Sexta-feira) - Horário: 16h00 - 17h30 - Sala: 7

Titanium with nanotopography inhibits osteoclast-induced disruption of osteoblast differentiation by regulating histone methylation
Bighetti-Trevisan RL, Almeida LO, Gordon J, Tye C, Lian JB, Stein GS, Rosa AL, Beloti MM
Biologia Básica e Oral - UNIVERSIDADE DE SÃO PAULO - RIBEIRÃO PRETO
Conflito de interesse: Não há conflito de interesse

The aim of this study was to evaluate the regulation of the osteoclast/osteoblast crosstalk by titanium with nanotopography (Ti Nano) and the mechanism involved in this process. Osteoblasts were cultured on Ti Nano and Ti Machined and osteoclasts into inserts in a co-culture model for 48 h, and non-cocultured osteoblasts were used as control. The RNAseq (DESeq2: FC>1.7; p≤0.05) detected that osteoclasts downregulated the expression of genes related to osteogenesis and upregulated genes related to histone modification and chromatin organization in osteoblasts grown on both surfaces. Osteoclasts also inhibited (p≤0.05) the gene expression of Runx2, Dlx5, Alpl, Ibsp, Bglap and Opg and protein expression of RUNX2 and ALPL, and such effect was reduced by Ti Nano. Regarding the mechanism, an increase in H3K9me2, H3K27me3 and EZH2 protein expression was observed on both Ti surfaces under coculture conditions (p≤0.05). ChIP assay revealed accumulation of H3K9me2 repressing the promoter region of Alpl and H3K27me3 repressing Runx2, Alpl, Ibsp and Opg in osteoblasts in the presence of osteoclasts, which were attenuated by Ti Nano. Immunofluorescence corroborated the ChIP assay, exhibiting less RUNX2 and ALPL and more H3K27me3 staining in osteoblasts under coculture conditions in a less pronounced way on Ti Nano.
In conclusion, Ti Nano may favor the clinical outcomes of implant osseointegration, since this nanotopography inhibited the osteoclast-induced disruption of osteoblast differentiation by reducing the accumulation of H3K9 and H3K27 methylated histones.
(Apoio: FAPESP   N° 2017/23888-8  |  FAPESP  N° 2018/17356-6  |  CNPq  N° 303464/2016-0)

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