HA006 - Hatton
Área: 4 - Odontopediatria

Apresentação: 10/09 (Sexta-feira) - Horário: 10h30 - 12h00 - Sala: 7

Selective Outcome Reporting (SOR) in Pediatric Dentistry restorative treatment Randomized Clinical Trials - A systematic Review
Elagami RA, Tedesco TK, Pannuti CM, Seabra G, Braga MM, Mendes FM, Raggio DP
Odontopediatria e Ortodontia - UNIVERSIDADE DE SÃO PAULO - SÃO PAULO
Conflito de interesse: Não há conflito de interesse

We aimed to evaluate the prevalence of SOR in the randomized clinical trials focusing on restorative treatment in primary molars. We searched for RCTs protocol registries in the ClinicalTrials.gov and WHO platform up to April/2021. We included any study classified as an RCT, with two or more experimental arms that evaluated any restorative treatment on primary teeth. The registries that did not result in any publications were excluded. Our search strategy identified 294 registries and 31 publications were included for data extraction. We have also calculated the median of journals' impact factors. Chi-square test was performed with 5% significance level. Our results found that the prevalence of SOR was identified in 19 papers (61.3%). The most common reason was a discrepancy in the primary outcome timeframe (48.4%). Twenty-six (83.9%) of the trials were registered retrospectively. Also, a discrepancy in sponsorship was identified in 22 (71%) of the trials. SOR was associated with the discrepancy in the follow-up period (p=0.003), with no association with registration timing (p=0.948). The impact factor of the journals ranged from 0.213 - 5.79. We found SOR in 9 (29%) of the six high impact factor journals and 10 (32.3%) of the eight with low impact.
Consequently, we emphasized the importance of transparency in RCT focusing on restorative care in children, including properly prospective registration and declaration of any discrepancy in the final manuscript, helping to avoid spin in science.
(Apoio: CAPES  N° 001)

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HA007 - Hatton
Área: 4 - Odontopediatria

Apresentação: 10/09 (Sexta-feira) - Horário: 10h30 - 12h00 - Sala: 7

Probiotic: in vivo evaluation of the effect on progression of periapical lesions, through microscopic analysis
Araujo LDC, Segato RAB, Messora MR, Furlaneto FAC, Salvador SLS, Nelson-Filho P, Pucinelli CM, Silva LAB
UNIVERSIDADE DE SÃO PAULO - RIBEIRÃO PRETO
Conflito de interesse: Não há conflito de interesse

The aim of the present study was to evaluate the effect of the probiotic Bifidobacterium animalis subsp. lactis (B. lacti) HN019 in vivo, as an irrigation solution for root canals in rats' teeth with induced periapical lesion. The animals were divided in four groups, as follows: group I: healthy teeth; group II: periapical lesion; group III: 2.5% sodium hypochlorite; group IV: probiotic, in 7, 21, 42 days. The probiotic irrigating solution was formulated by adding the probiotic at 2.7x 10⁹ CFU in water medium and 2.0% of carboxymethylcellulose. The root canals were irrigated on day 0 and days 3, 7, 14, 21, 28 and 35 days, after periapical lesion induction. The mandible samples were stained in HE and analyzed under optical microscopy to describe the characteristics of the pulp tissue and the apical and periapical regions, in parallel with the morphometric analysis of the extension of the lesion area, osteoclast marking (TRAP), immunohistochemistry to identify markers of the osteoclastogenesis (RANK, RANK-L and OPG) and microbiological analysis using the Brown & Brenn technique. The data were analyzed using software R, version 4.0.0, applying SAS 9.4 and Graph Pad Prism 5, with a significance level of 5%. The histopathological, immunohistochemical and microbiological analysis parameters in probiotic groups were significantly better when compared to groups without periapical lesion and 2.5% sodium hypochlorite (p<0.05).
Therefore, probiotic demonstrated a therapeutic potential on the progression of periapical injuries.
(Apoio: FAPESP  N° 2018/22038-3)

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HA008 - Hatton
Área: 4 - Ortodontia

Apresentação: 10/09 (Sexta-feira) - Horário: 10h30 - 12h00 - Sala: 7

A microtomographic and histological analysis of the induced orthodontic movement in obese rats
Horta KC, Paula-Silva FWG, Stuani MBS, Elias LLK, Flores Mir C, Graf D, Consolaro A, Matsumoto MAN
UNIVERSIDADE DE SÃO PAULO - RIBEIRÃO PRETO
Conflito de interesse: Não há conflito de interesse

To evaluate the effect of obesity over induced tooth movement (ITM) through computerized micro-tomography and histological analyzes in obesity-induced Wistar rats. Forty rats of approximately 125 g were randomly assigned into four groups according to obesity status and ITM duration (mesial movement of the left maxillary first molar for seven and fourteen days). Eighty hemi-maxillas were processed for histological and micro-CT examination. ITM was measured in the micro-CTs. The number of osteoclasts was analyzed through staining of enzyme tartrate-resistant acid phosphatase. Also, the presence or absence of focal hyalinization (FH), frontal bone resorption (FBR), and active root resorption (ARRC) were evaluated in sections stained with Hematoxylin-Eosin. Data were submitted to appropriate statistical analysis using Graph Pad Prism 5.0 and Stata13 (α=0.05) Obese animals showed higher rates of ITM after seven (p=0.037) and fourteen days (p≤0.001) than non-obese animals. A higher quantity of osteoclast was observed after seven days of ITM in obese animals (p=0.043). Low frequencies of FH were observed after seven days of ITM. After seven days of ITM high frequency of FBR and low frequency of ARRC were found in obese animals, while, medium frequencies of FBR and ARRC were seen in non-obese animals. Low and medium frequencies of FBR were observed in non-obese and obese animals respectively after fourteen days of ITM.
Obese rats showed significantly higher rates of molar movement and different morphological responses in the surrounding bone and periodontium to ITM.
(Apoio: CAPES  |  FAPESP)

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HA009 - Hatton
Área: 5 - Materiais Dentários

Apresentação: 10/09 (Sexta-feira) - Horário: 10h30 - 12h00 - Sala: 7

Self-assembling peptide-containing electrospun scaffolds for mineralized tissue regeneration
Araújo IJS, Ferreira JA, Daghrery A, Andrade JSR, Puppin-Rontani RM, Bottino MC
Odontologia Restauradora - FACULDADE DE ODONTOLOGIA DE PIRACICABA
Conflito de interesse: Não há conflito de interesse

Combining scaffolds and bioactive molecules to mimic the extracellular matrix (ECM) is vital for mineralized tissue regeneration strategies. Electrospinning allows the fabrication of ECM-like scaffolds. Meanwhile, self-assembling peptide (SAP) P₁₁-4 is applied to repair early caries lesions. Thus, to explore SAP effectivity on mineralized tissue regeneration, distinct concentrations of P₁₁-4 were adsorbed in electrospun scaffolds. After scaffolds immersion in simulated body fluid, mineral precipitation was verified by Scanning Electron Microscopy (SEM). FTIR and XRD detected functional groups and crystalline phases, respectively. Cytocompatibility, mineralization, and gene expression assays were conducted using stem cells from human exfoliated deciduous teeth. In vivo bone regeneration was assessed by defects in rat calvaria. ANOVA and Tukey posthoc tests verified differences for cytocompatibility, mineralization, gene expression, and in vivo regeneration (α=0.05). SEM images indicated mineral precipitation. FTIR and XRD confirmed apatite-like formation and crystallization induced by SAP-containing scaffolds. SAP-containing scaffolds were cytocompatible, stimulated cells to mineralization, and upregulated bone-related genes' expression. Also, substantial in vivo bone regeneration was induced after eight weeks. Histological analyses evidenced homogeneous bone formation.
Finally, SAP-containing scaffolds are promising bioinspired constructs for mineralized tissue regeneration in the oral and craniofacial complex.
(Apoio: CAPES  N° 88887.368381/2019-00  |  NIH  N° R01DE026578)

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HA010 - Hatton
Área: 5 - Dentística

Apresentação: 10/09 (Sexta-feira) - Horário: 10h30 - 12h00 - Sala: 7

Dental trauma splints for mixed dentition - A finite element analysis of splint material, extension, missing teeth, and PDL representation
Vilela ABF, Soares PBF, Versluis A, Soares CJ
Dentística - UNIVERSIDADE FEDERAL DE UBERLÂNDIA
Conflito de interesse: Não há conflito de interesse

This study evaluated the effect on the mobility of splint materials and extensions for avulsed central incisor, stabilized with and without the adjacent incisor under horizontal and vertical loading with different periodontal ligament (PDL) conditions. Seventeen 3D models were created from a CBCT of a 7-year-old patient without erupted permanent upper lateral incisors. A new method was created to simulate the avulsion injury, 1000N palatal load was applied on the central incisor creating increased alveolus with 0.45mm of PDL thickness. Wire-composite splints with 0.9mm (WCS) or 1.0mm nylon-composite splints (NCS) were created with and without the adjacent upper central incisor and four splint extensions were simulated. PDL conditions were investigated, simulating liquefied PDL or PDL with silicon. Mobility was calculated under horizontal, intrusive and extrusive loading. The NCS allowed higher mobility than the WCS, irrespective of extension, PDL or load conditions. During horizontal loading, silicon-like PDL allowed around 0.2mm mobility of the avulsed tooth with the WCS, similar to the intact tooth, whereas liquefied PDL allowed 25% higher mobility with a WCS than NCS.
A 1.0mm NCS seems to be a good option for splinting avulsion injuries during mixed dentition. The NCS provided flexibility for PDL healing while maintaining stability, even when missing adjacent teeth. Extensions beyond directly adjacent teeth did not alter mobility with the NCS, but should still be considered extra protection in case of bond failure or exfoliation.
(Apoio: CAPES  N° 001  |  FINEP  N° FAPEMIG  |  CNPq)

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